Sentence examples for template for mutation from inspiring English sources

The fragment was subcloned into the pUC18 vector between the EcoRI and XbaI restriction sites to produce pUC18-FPR1, whish is the template for mutation analysis.

The most well-known NGS technologies have been developed by Illumina (and Solexa, Inc, purchased by Illumina in 2007; San Diego, CA, USA), Helicos BioSciences (Cambridge, MA, USA), ABI/SOLiD, and 454 Life Sciences (a subsidiary of Roche; Branford, CT, USA) and use a single-molecule template for mutation detection with cloning-free approaches.

The total number of templates for mutation is 69 repeats in the sequenced S strain.

Aliquots of each diluted DNA (2 μl) were transferred into 96-well master plates AB-06000, ABgene, Rochester, NY) and served as the templates for mutation screening.

The plasmid with the rs9383935 G allele was used as the template for the mutation G → A.

As 'alternative transcript 1' was found to be the more abundant transcript, we used it as the template for our mutation screening experiments.

A portion of the genomic DNA was amplified by REPLI-g® Screening Kit (QIAGEN) and was used as template for the mutation screening.

The U-A-U-A-U pattern may provide an ideal template for testing mutation effects on both transcription termination and poly(A) site selection.

The plasmid pH5c- cdc8 + (Cranz-Mileva et al., 2013) is the template for introducing mutations into cdc8.

Briefly, the plasmid pAdCMV5cTA was used as a template for CymR mutations and the PCR was performed with the primers 5'-TCCACTTTGCCTTTCTCTCC (forward primer) and 5'-GTTTTTCGTACGCGC GCGGCTGTACG (reverse primer) under conditions which would lead to frequent misincorporation of nucleotides.

A double mutation was achieved using the mutated Cys286 DNA as a template for introducing a mutation at Cys295 to alanine.