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Moreover, DNA is more stable in a buffer with pH above 7, but the DNAs repeated freezing and thawing or storage in distilled water are not fit as template for long PCR [ 10, 22].
BAC DNA (1 μg) was linearized with NotI or 1 μl BAC DNA was used as template for long PCR amplification using primers 5′C-strain_T7_Not1 and 3′CSFV (Additional file 1: Table S1).
Crude extracted DNA from a tiny amount of animal tissue was used as template for long PCR amplification that nearly covered an entire mitochondrial genome (see Additional file 1: Table S1 for primer sequences used for the long PCR amplifications for each taxon).
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Problems with prevalence rates notwithstanding, incidents of child sexual abuse before age 18 tend to form the template for long-term effects that extend beyond the personal difficulties of the survivor and influence future relationship dynamics [ 21].
BACs to be used as templates for long PCR or for screening by restriction enzyme digestion were purified from 4 ml overnight cultures of E. coli DH10B using the ZR BAC DNA Miniprep Kit (Zymo Research).
On its first album, Santana showed more of its beginnings as a blues band than it would in later years, but the album set the template for a long career to follow: conga-driven instrumentals that surged behind the jabs and wails of Carlos Santana's guitar solos, like "Jingo" and "Soul Sacrifice," and radio-ready songs about the perfidy of women like "Evil Ways".
Designers have borrowed its contours as a template for as long as ufologists have reported sightings.
Walcott demonstrated his recent fine form on the right flank and England embraced their manager's willingness to move even further away from the 4-4-2 formation that was his template for so long.
Our template for life-long love is fractured, so we understand the transitory nature of relationships more than ever.
In this study we tested template mtDNA amounts between 1000 and 10000 GEs that turned out to cover an appropriate template amount range for long PCR fragments.
Therefore, it is vital to carefully control the procedure of removing the polymer fiber templates for fabricating long, continuous, and porous metal oxide submicro- and nanofibers.
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