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All PCR constructions used 26695 genomic DNA as template for initial amplifications.
The original repressilator plasmid, pZS1 (Elowitz and Leibler, 2000) was used as a template for initial characterization and for construction of the OR2* mutant.
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This PCR method may be useful for obtaining templates for initial sequencing of novel orthoreoviruses from diverse vertebrate hosts.
The template for the initial amplification reaction was a 5'-STRETCH plus cDNA library cloned in lambda gt10 vector (Clontech BD Biosciences, Mississauga, ONT).
For the crystallographic analyses of TEAS W273E and farnesylated TEAS W273E, the structure of isomorphously crystallized, wild-type TEAS (with residue 273 truncated to CB) provided a template for the initial atomic model, which was further refined (phenix.refine) in Phenix.
Finally, the derived sockets are employed as eye estimates in which DAISMI is employed to detect the stable approximate pupil-center position by averaging the positions of eyeball border vertices which is necessary for initial template orientation of DTBGE and the final movement displacement vector.
DAISMI finds the stable approximate pupil-center location by calculating the mass-center of eyeball border vertices to be employed for initial deformable template alignment.
And to be fair, it was actually Terry J who established the initial template for my contribution to the group that eventually became Monty Python.
Michael Hertz, whose firm is credited with designing the initial template for the map, has long chafed at Mr. Tauranac's calling himself the "design chief" on a project that has garnered numerous accolades, including a commendation from the United States Department of Transportation and the National Endowment of the Arts.
The ligation product was directly used as a template for the amplification of initial ribosome display library.
The Gal4-luciferase vector (pFRluc, Stratagene) was used as an initial template for construction of the reporter vector.
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