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This work supports the use of a utrophin template for gene or protein therapy designs.
Non-segmented negative-strand RNA viruses all possess an RNA genome encapsidated by nucleoprotein and other viral factors to form a nucleocapsid, which directly serves as the template for gene transcription and genome replication by the viral RdRp (Ruigrok et al., 2011; Zhou et al., 2013).
Alternatively, if the right TIR is used as template for gene conversion, then the left TIR becomes identical to the right TIR (Fig. 3e).
If the left TIR is used as template for gene conversion, then the right TIR becomes identical to the left TIR (Fig. 3d).
Despite extensive divergence between these ancient paralogs, their distant sequence homology appears to have provided a template for gene conversion (fig. 3 a and b).
This may have implications in terms of using rice as a template for gene discovery and gene function prediction in the larger genome monocots.
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Reverse-transcribed RNA (0.5 mL) was used as PCR template for gene-specific primers (Additional file 1: Table S3) (Ren et al., 2013).
For example, the increase in nucleosome density caused by tethered HIRA may be accompanied by a decrease in the average linker length; and, if linker regions are preferred templates for gene conversion, shortening of those regions may contribute to decreased gene conversion tract length in the HIRA transfectants.
Gene prediction was undertaken following the MAKER pipeline [ 25] using PST ESTs, de novo transcript assemblies generated in this work (see below), and PGT and P. triticina (PT) peptide sequences as templates for gene model discovery.
Glaucophyte cDNAs (Cyanophora paradoxa NIES-547, Gloeochaete wittrockiana SAG 46.84 and Cyanoptyche gloeocystis SAG 34.90) were prepared as described in the previous study [ 19], and used as templates for gene isolation.
The results obtained using these novel approaches provide new insights into template design for gene correction and the relationships between multiple DSBR pathways at a single endogenous disease gene.
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