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Mr. Coleman said the creation of a special improvement district 12 years ago, a joint venture of the City Council and the neighborhood, bolstered development and has provided the city with a template for four other similar districts.
After the prescribed induction time, a 1 ml sample of the culture was centrifuged (15,000×g, 1 minute), and the pelleted culture was used as the template for four PCR reactions ("Culture PCR").
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When did you imagine using it as a template for one of your own?
The Schubertiade became a sort of template for "Three Pianos," and while the three men re-enact their own often-disputatious personalities in the play, they also assume the roles of Schubert and his friends.
As an early ski instructor in Aspen, Colo., Obermeyer realized that his comforter stuffed with goose feathers, packed by his mother when he left Germany for North America, could provide the template for one of the first down parkas.
This detection scheme is based on luminescent resonance energy transfer between upconversion nanoparticles and the intercalating dye, SYBR Green I. Target DNA serves as a template for two DNA probes, one of them covalently attached to upconversion nanoparticles, to be joined into a long, hairpin-forming DNA by ligase.
With the Beverly Hills boutique set to serve as a template for two additional U.S. stores that are scheduled to open before year's end — in Bal Harbour, Fla., and New York City — and the label on the cusp of its 20th birthday, does this mean the brand known for a witty blend of naughty and nice is toning down and growing up?
All 96 first round reactions for each time point were pooled and used as template for three inner PCR reactions.
Plasmid pET15b/eIF4AIIP was used as template for two PCR reactions, using either (i) primers DraIII Forward and T Reverse and (ii) T Forward and BamHI Reverse.
The resulting cDNAs were used as template for two round PCR as described above for whole blood FV gag amplification.
The linker ligation was used as a template for two rounds of PCR to amplify the transposon/genome junction.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com