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The silicone surfactant serves as the template for directing meso-structures, the source of functional groups for surface modification, and the silica source for strengthening the wall of mesochannels.
During pyrolysis, the intermediate, g-C3N4, derived from the condensation of dicyandiamide, played two important roles, namely, acting as the thermally decomposable template for directing the formation of carbon nanoflakes and serving as an in situ N source upon decomposition.
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Improved scaffold mechanical properties along with ECM alignment with time in culture suggest this multiporous silk construct as a useful micro-patterned template for directed tissue growth with respect to form and function of meniscus-like tissue.
The Arg variant (Oluc-N166R) was most improved (∼50% increased stability at 37 °C and ∼3-fold higher luminescence intensity) and was used as the template for directed evolution.
A plasmid expressing protein GLE1128A was constructed similarly, using pET-R3GL as the template for directed mutagenesis with primers Pexp_E1228A1 and Pexp_E1228A2, changing codon GAA (Glu) to GCA (Ala).
The dimensions of the structures are 0.43 ± 0.15 μm in diameter and they have then subsequently been used as templates for directing the synthesis of silica silver and silica polyanthranilic composite hollow spheres.
In summary, we have synthesized flexible chiral cyclodextrin-based templates, and found that they are as effective as rigid templates for directing the synthesis of porphyrin nanorings.
The discovery that strict preorganization is not necessary will make it easier to design templates for directing the formation of new macrocycles.
Since oligonucleotides have become popular as templates for directed changes following induction of a DSB by Cas9 and other gene editing tools [ 2, 31] we sought to determine if NU7441 or KU-0060648 could also stimulate HDR mediated by these much shorter, single-stranded DNA templates.
Although the first Plowshares had been a disappointment, a new template for direct action had been created — one that inspired more than a hundred similar break-ins.
These samples were used as a template for direct DNA sequencing.
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