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For qPCR, a mixture of all cDNAs was used for all treatments, as a template for calibration curves designed for each pair of primers.
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The full-length cDNAs were subcloned into pCR4Blunt-TOPO vector (Invitrogen, Carlsbad, CA) and the resulting plasmids were used as templates for the calibration controls of real-time PCR reactions.
Three-fold serial dilutions were made from this mixture as templates for the calibration curve.
The DNA templates for the calibration lepB mRNAs (cWT and cMT) were ordered as GeneART oligos (Invitrogen) and cloned into pCR-Blunt II-TOPO vector according to the Zero Blunt TOPO kit protocol (Invitrogen, Carlsbad, CA).
Fig. 6 Positioning template for the 137Cs source Fig. 7 Setup used for calibration with circulating FDG Fig. 8 Setup used to extract time-activity curves during clinical PET studies.
The proposed method does not require exact template matching, rather it has the capability to classify and use only suitable data points for calibration.
The nuclear material was intended for scientific research and for calibration of radiation measuring equipment.
3. Mistakes are tools for calibration.
"Different pointings for calibration fields won't be necessary".
For calibration, local regression is performed.
For calibration, local regression is performed [50].
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