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Exact(5)

The register of the nucleic acids could be defined by bromine labelling of the template except for the structure containing 4 nt RNA.

As a negative control, a pCRISPomyces-1 derivative plasmid bearing all CRISPR elements (tracrRNA, redN spacer, and redN editing template) except for the cas9 gene was constructed and conjugated into S. lividans.

For the competitive PCR, each template (except for the references) was mixed with its appropriate competitor array and amplified by PCR by using 40 cycles at 94°C for 10 s, 56°C for 20 s, and 72°C for 30 s.

In a total volume of 25  μL, each RT-PCR reaction contained primer pair mix (Invitrogen), 12.5  μL of 1x iQ TM SYBR Green RT-PCR buffer, nuclease-free water (Ambion), and 5  μL of cDNA (2 ng/ μL) as a template, except for negative controls.

On the basis of guidelines requiring at least 10 events per predictor variable in a regression model, 22 the expected number of events was adequate for the number of variables recorded in the template, except for the much rarer outcome of bacteraemia.

Similar(55)

Case 3 template extraction, template deployment except for volumes, tester resources preparation, environment info acquisition, patch distribution, test cases execution, virtual resources deletion except for volumes.

Case 3: template extraction, template deployment except for volumes, tester resource preparation such as Internet connection settings, environment information acquisition, patch distribution, test case execution, virtual resource deletion except for volumes.

(We consider the case where service providers replicate virtual resources beforehand and do not delete them after verifications) Case 3: template extraction, template deployment except for volumes, tester resource preparation such as Internet connection settings, environment information acquisition, patch distribution, test case execution, virtual resource deletion except for volumes.

Similar trends of results were observed with four DNA lesion templates, except for the case with the template N-EtG and the R96G variant.

Uniform PCR products of the expected size were obtained in all reactions with genomic DNA templates, except for mouse exon 1; these products were all gel purified, cloned and sequenced.

By taking extra caution, we carried out MSP of all the targets with the M. Sss I treated normal liver DNA as positive control templates, except for the MAGEA1 gene was unmethylated in the normal liver tissue.

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