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Filtration experiments showed that the imprinted hybrid membrane was able to adsorb higher amounts of template even in non-equilibrium dynamic binding conditions.
However, the possibility mentioned in this comment that free NP sequesters the viral RNA template can be ruled out due to the fact that robust ApG-primed RNA products were synthesized from the exogenously-added viral template even in the presence of the same amount of NP present in the mnRNP preparation used for our experiments (see lanes 1 and 6 in Figure 3B).
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"The Cadillac of X" has also survived as a sturdy phrasal template, even if it's now often used with tongue in cheek.
Because it is sometimes very difficult to determine which atoms in the catalytic site should be included in the templates, even experts on enzyme structure and function might have to create the best template through trial and error.
The HCV example demonstrated that rhPCR improves reaction specificity by preventing primer-dimer formation (a template independent event), even in a system highly prone to this problem.
Their repetitive arrangement within the genome provides excessive amounts of template DNA for PCR, even in smallest organisms.
However, strict isolation of the production and manipulation of high-titre plasmid stock solutions and common good laboratory practices when handling plasmid solutions at low concentration (e.g. below 1,000 copies per microlitre) should allow the use of plasmids as a source of a suitable DNA template in PCR analysis, even in routine analysis.
In summary, we have achieved length uniformity of template-assisted electrodeposited nanowires over 95%, even in highly disordered commercial AAO template, when the deposition temperature was lowered down to subzero centigrade degrees.
We were surprised to find that the background of certain miRNAs was very high in the RT-qPCR results, even in no-template-controls during practice trials.
If the amount of DNA proves insufficient to type all SNPs independently, it will be possible to apply MDA even in LCN templates.
The interactions between the CC and LF of pol η form a "molecular splint" to hold the template strand in the B-form even in the presence of a UV cross-linked cis-syn pyrimidine dimer.
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