By adding the training images directly, one can obtain a composite template with high discrimination ability and robustness for face recognition task.
Initial assay performance was demonstrated using a wild-type amplicon, including most of the HIV-1 pol gene, comprising protease (PR), reverse transcriptase (RT) and integrase (IN) coding sequences, as the template DNA for ligase discrimination.
After the newly synthesized strand is copied, the primer forms a perfect match with the template and no further discrimination can be achieved.
However, they never investigated whether the question stem templates order the auto-generated questions according to their easiness in Bloom's taxonomy or whether the question stem templates affect the questions' discrimination.
However, the use of templates can improve the discrimination between the two different tissue types by considering the location as a feature.
In agreement with the observed role of R110 in specific recognition of A+3, a Brf2 R110A mutant displayed a reduced affinity for DNA compared to Brf2 wild-type, an effect that was most prominent in presence of an A nucleobase at position +3 of the template strand, suggesting a functional discrimination between T′+3 -containing and T′+3 -less promoters.
It is more likely that an inherent feature of the Illumina platform, such as PCR-based cluster generation, is responsible for discrimination against sequencing templates that are >90% AT-rich (Bartfai et al., 2010).
This PCR product (2 μl) was then used as template for genotyping using custom allelic discrimination TaqMan® assays developed for each mutation.
10 ng DNA was used as template for 2 μl TaqMan allelic discrimination assays (Applied Biosystems, Foster City, CA, USA) performed on the ABI 7900HT platform in 384-well format and analysed with Autocaller software, as previously described [ 22].
We reasoned that mutating G498 into a sterically more demanding amino acid would result in a more crowded template binding site, which might effect discrimination between C and 5mC upon extension from mismatched primer strands.
