As a tactical-level plan, a yard template determines the assignment of spaces in a container port yard for arriving vessels.
Theoretically, a template determines the size, shape, and distribution of nanoparticles[32].
PSi materials are termed "top-down" materials as opposed to the synthesized mesoporous molecular sieves, which are so called "bottom-up" silica materials that refer to the self-assembly of silicon oxide by means of polymeric templates determining the structure obtained.
Single forward and reverse PCR primers were selected with full complementarity to the template determined by sequence analysis.
Amplification of Vpr from a non-infectious clone, pBKBH10S, was performed using single forward and reverse primers complimentary to the template determined by sequence analysis.
As a simulation progresses the template reflects more and more a biased overlay between the templates determined by the output activation of the template units.
We used Flavivirus genus-specific primers (6 ) to perform reverse transcription-PCR amplification using viral genomic RNA as a template and determined the nucleotide sequence of the virus from the amplified cDNA fragment.
To control for preexisting mutations present within the DNA synthesis template, we determined the HSV-tk mutation frequency for each ssDNA by electroporation of FT334, followed by selective plating on media containing 250 μg/ml carbenicillin in place of chloramphenicol, with or without FUdR.
To control for pre-existing mutations present within the DNA synthesis template, we determined the HSV- tk mutation frequency for each ssDNA by electroporation of FT334, followed by selective plating on media containing 250 µg/mL carbenicillin, with or without FUdR (Eckert et al. 2002a).
Standard PCRs were run with fivefold dilutions of the cDNA template to determine the optimal amount of template and optimal annealing temperature for the experimental and reference reactions, using 500 nM of each primer.
