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Single forward and reverse PCR primers were selected with full complementarity to the template determined by sequence analysis.
Amplification of Vpr from a non-infectious clone, pBKBH10S, was performed using single forward and reverse primers complimentary to the template determined by sequence analysis.
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As a simulation progresses the template reflects more and more a biased overlay between the templates determined by the output activation of the template units.
Through providing the various driving potentials from −25 V, −10 V, −5 V to −2.5 V, the different mechanism of electrophoretically depositing ZnO nanoparticles into the colloidal crystal template was determined by the SEM observation of the filled templates.
The optimal position of the template is determined by {k}_{mathrm{opt}}=underset{k}{arg max}left(c k)right) (9).
where (x i, y i )(i = 1, 2, …, N t ) denotes the original template coordinates determined by key KEY t 2, (cx, cy) is the geometrical center, and round is the rounding operation.
For each sample, the relative amount of starting template was determined by calculating the ΔΔCt after correcting the Ct values for expression of RPL4.
Quantitative measures of DNA template quality determined by QFI-PCR were then compared with targeted NGS results using defined metrics across varying 'functional' FFPE DNA copy numbers to demonstrate DNA quality and input thresholds that supported accurate variant calling.
For a target FFPE DNA sample, 5 ng of template (nominally determined by NanoDrop) was input into QFI-PCR, and the Cq output was converted to a 'functional' copy number.
The number of ng amplified by the allele specific primers in the presence of the target template was determined by referencing a standard curve presented in Figure 2. The number of amol was calculated from the ng of DNA derived from the standard curve and corresponding length of coding region (Table 1).
The amount of carbon templates was determined by 5 wt% weight ratios of those to theoretical yield of SAPO-11.
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