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Moreover, maximum weight loss of approximately 25% was observed due to the template degradation.
Pause-induced donor template degradation initiates strand transfer.
All of these were retrieved from long-term storage, and template degradation was a significant possibility.
The hairpin structure facilitates RT pausing, which stimulates RT-RNase H activity and results in donor template degradation.
In both sets of experiments the absence of specific bands after 48 h corresponds to the end of blood meal digestion, indicating likely template degradation by digestive enzymes.
Template stability and the lack of uniformity in DNA template concentration are also a concern, particularly as template degradation during blood digestion has been observed for mosquitoes (Mukabana et al., 2002; Ngo and Kramer, 2003; Kent and Norris, 2005).
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To prove this statement we exploited EGTA to prevent template DNA degradation in energy-depleted reactions.
In recent years, several methodologies have been published and claimed to be suitable for large population screening [ 22, 30, 45- 50], although all have presented some technical and non-technical problems, including the amount of DNA template required, degradation due to the use of bisulfite, inclusion of females, and failure to detect unmethylated expanded alleles.
Consistent with the data obtained with synthetic DNA templates, the degradation of ssDNA oligos by PDEI also led to the suppression of ATM activation induced by chromosomal breakage.
High recombination frequencies are assumed to be related to the presence of partial cDNA that results from incomplete cDNA synthesis as well as partial RNA templates caused by degradation.
For the nasal class, there were small phone accuracy gains, and for silence, there were small accuracy degradation by template matching, but both changes were small and insignificant.
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