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All multiplex pyrosequencing signals generated for TEM and SHV were analyzed using a new web interactive application (available at https://ucl-irec-ctma.shinyapps.io/Pyrosequencing-TEM-SHV and in 'download' section of http://www.uclouvain.be/ctma.html where a template data set can be downloaded) written with R.3.1.2 software (Ihaka and Gentleman 1996) using the "shiny" package.
For each transcript, the same number of reads as in the template data set was generated by the simulator.
At 2 units of ligase enzyme, MFI values for the test panel were generally lower for 4 μL of DNA template than for 2 μL of DNA template (Data set S.5), which might be explained by inhibition of the ligation reaction because of impurities in the DNA extract.
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Evening information and education sessions by relevant specialists were held at regular intervals to advise local GPs of the new referral template and minimum data set.
Empirically, substituting sample-specific templates for the reference templates in the current data set does not substantially impact any of the reported relationships to cognition.
Therefore, we developed a referral template containing a minimum data set for each of the specialties.
The Excel data template with its resultant data set was further processed by an anonymizer, which substituted a code number for the locally available surgical accession number.
This template was developed and amended through careful re-reading of all the interview transcripts and the application of successive drafts of the template to the full data set.
Pasaniuc et al. (2010) select an imputation template from an external data set based on similarity between haplotypes in the external data set and each individual haplotype in the study sample.
We measured network connectivity using the Template-Based Rotation (TBR) method (Schultz et al., 2014), in which cortical network parcellations are defined from a reference data set and applied as templates to a target data set.
The Sherpa load_template_model function, an extension of load_table_model (see 'ahelp load_table_model'), can be used to read in a collection of template models from a directory full of template files, in order to compare a data set to all of the templates in that collection.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com