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The propensity for repeat array deletions and their orientation dependence have most frequently been explained by the presence of single-strand DNA on the lagging-strand template coupled to the greater stability of CTG repeat hairpins that are likely to provide a good template for replication slippage.
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For each binding reaction, 1 pM biotinylated DNA template was coupled to 50-μg streptavidin-linked magnetic beads (Dynabeads M-280 Streptavidin; Invitrogen, Carlsbad, CA, USA).
Many studies and reviews on the topic of neurodegenerative diseases with LBs are organized as if "molecular template" for αS and its spread along "structural template" are unequivocally coupled to provide a direct mechanism to explain the progressive clinical and pathological nature of these disorders.
NMR exchange spectroscopy (EXSY) experiments show that these complexes exhibit correlated motion, in which the conrotatory rotation of the two template wheels is coupled to rotation of the nanoring track.
Stimuli-responsive polymer microgels can be produced with exquisite control using droplet microfluidics; however, in existing methods, the droplet templating is strongly coupled to the material synthesis, because droplet solidification usually occurs through rapid polymerization immediately after the microfluidic droplet formation.
HB-19 presents pentavalently the pseudo-tripeptide Lysψ(CH2N -Pro-Arg CH2N -Pro-Argpeptide template.
The data were coupled to the array template using the GeneChip Operating System (GCOS) software, V. 1.4.
Digested linker-ligated DNA was used as a template for PCR amplification (20 cycles) and coupled to fluorescent dyes.
The mechanism of poliovirus RNA recombination has been shown previously to be coupled to RNA replication, presumably by template switching during the replication of parental RNAs.
Presentation is directly coupled to content – if you update or delete a template, nothing else is affected.
The strategy to prepare Pd nanowires is based on ion-track template coupled with electrochemical deposition, which is described in detail elsewhere [31, 32].
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