Sentence examples for template corresponding from inspiring English sources

Arenaviruses use RNA as their hereditary material; RT-PCR creates a DNA template corresponding to viral RNA genes.

From this result, we observed that our approach is able to generate a chemical template corresponding to the original chosen molecule.

Instead of directly finding a minimal local value in the first derivative curve, which is often difficult due to its great sensitivity to noise, a hybrid dynamic template corresponding to a typical derivative variation in the region around the outer lower lips position is first established.

Dig-labelled probes for sense and antisense Pomc mRNA were generated from the cDNA template corresponding to exon3 of Pomc in a pGem- T vector (Promega) and transcribed by SP6 or T7 RNA polymerase (Progema).

The reaction was performed in 12.5 µl consisting of 6.25 µl 2xSYBR Green Master Mix (Applied Biosystems, Foster City, CA, USA); 2.5 pmol of each primer; 2 µl 1∶10 dilution of cDNA template (corresponding approximately to 4 ng RNA).

Each reaction (final volume, 20 µl) contained 10 µl 2×SYBR® qPCR Mix (TOYOBO), 0.04 µl 50×ROX reference dye, 1 µl of each the forward and reverse primers (0.25 µM), 2 µl of the cDNA template (corresponding to 50 ng of total RNA), and 7 µl of RNase-free water.

The computational efficiency of our framework was demonstrated with the full-sequence design of the peptide with flexible backbone templates, corresponding to the mutation of all positions except the native cysteines.

As I described above, RT-PCR creates DNA templates corresponding to viral RNA, copying them many, many times, so that even if there is only a small number of genes in a sample, they can be detected and isolated for study.

Four double-stranded DNA templates corresponding to each of the four zipper peptides were assembled with pairs of oligonucleotides, which formed partial duplexes upon annealing via complementary sequences (see Table S1; underlined nucleotides are complementary).

Sequence verified riboprobe generation templates corresponding to bps 884 1238 of Gapdh (Accession # NM_001001303) and bps 294 705, 792 1185 and 294 1185 of Il7 (Acc # NM_008371) were produced via a PCR based strategy that utilized forward primers flanked by the T3 promoter and reverse primers flanked by the T7 promoter.

Radiolabelled probes were generated using random hexamers on PCR-generated templates corresponding to internal regions of the relevant genes.