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The specificity of the SYBR I qPCR method was further confirmed by the fact that the study identified less positive samples than conventional PCR when applied to PCV2 test, as well as that it preferred to amplify P1 genome when DNA template contains both P1 and PCV2.
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Since we are using a nucleosomal template containing both LexA and Gal4 binding sites, chimeric proteins containing a LexA or Gal4 DNA binding domain can be used and fused to distinct peptides or protein domains that are known to interact with a particular chromatin modifying enzyme.
The obvious advantage of using a template containing both LexA and Gal4 binding sites is that co-activator molecules can for example be recruited via Gal4 fusion proteins, such as the commonly used Gal4-VP16 molecule, whereas LexA fusion proteins, such as LexA-Mad, can be used to recruit co-repressor molecules.
Again, we used non-methylated and methylated DNA templates containing both rsd promoters P1 and P2 for gel retardation.
These templates contain both T7 promoter sequence and polyA sequence.
If the template contains guide atoms, they are stripped out.
All neighboring blocks template (AN): This template contains 8 blocks × 16 pixels =128 pixels.
Empty cross template (EC): This template contains 4 blocks × 16 pixels =64 pixels.
Four diagonal blocks template (4D): This template contains 4 blocks × 16 pixels =64 pixels.
The refinement templates used along the various refinement passes are labeled as follows: Empty cross template (EC): This template contains 4 blocks × 16 pixels =64 pixels.
If there is one subject atom, and the template contains one guide atom, and the guide atom has one adjacent neighbour, this primitive applies.
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