Sentence examples for template containing two from inspiring English sources
A DNA template containing two adjacent BrdU residues (CBB template) was annealed with various DNA primers (Figure 1A) to prepare a series of P/Ts that were chain-terminated at different positions relative to the BrdU residues.
DNA fragments (597 bp) were generated by PCR, using a 5′ biotinylated oligonucleotide primer on a plasmid template containing two non-overlapping KOPS pointing towards the biotinylated end.
74mer tRNA3 tRNA was in vitro transcribed from a DNA oligonucleotide template containing two 5′ methoxy (C2′ position) nucleotides to site-specifically truncate transcription by the T7 MEGAshortscript kit (Ambion).
This sequence is compatible with a circular DNA template containing four entire ORFans (191 194).
To test whether H3K64ac can indeed promote histone displacement from chromatin, we assembled chromatin with unmodified H3, H3K64R, or H3K64ac octamers on a template containing five GAL4-binding sites in front of a MLP promoter and performed a histone eviction assay.
Among the latter, the term regulation of transcription, DNA-templated contains nine of the 15 transcription factors overexpressed in the yeast condition (Table S5).
The LUT information for each template should contain two parts, the template index and the predicted gray value,.
Four modified synthetic SMRTbell templates were made, each containing two 5mC, 5hmC, 5fC or 5caC modifications.
The pSSU- alt-2 construct (see above) was used as the template in a PCR reaction containing two oligonucleotides: 5'-TGATTCTGATACACACGGGAGTGT-3' (antisense, primer phosphorylated) and 5'-TATGTAACCAAAGGGAATTTGTT-3' (sense).
The genomic DNA was used as template in a PCR reaction containing two primers annealing on the DMI1 coding sequence (DMI1_for: 5′-ATCCTTGGCTGGAGTGACAAATTG-3′; DMI1_rev: 5′-CTGATCTGCATTTTCGTCCGCAGC-3′) and a third primer complementary to the Tnt1 sequence (Tntail1: 5′-TATGCAAAGAACTTGTCGGCATGC-3′) [ 49] (Additional file 3).
The template Z contains two segments.
