Sentence examples for template containing both from inspiring English sources

The obvious advantage of using a template containing both LexA and Gal4 binding sites is that co-activator molecules can for example be recruited via Gal4 fusion proteins, such as the commonly used Gal4-VP16 molecule, whereas LexA fusion proteins, such as LexA-Mad, can be used to recruit co-repressor molecules.

Since we are using a nucleosomal template containing both LexA and Gal4 binding sites, chimeric proteins containing a LexA or Gal4 DNA binding domain can be used and fused to distinct peptides or protein domains that are known to interact with a particular chromatin modifying enzyme.

The specificity of the SYBR I qPCR method was further confirmed by the fact that the study identified less positive samples than conventional PCR when applied to PCV2 test, as well as that it preferred to amplify P1 genome when DNA template contains both P1 and PCV2.

Again, we used non-methylated and methylated DNA templates containing both rsd promoters P1 and P2 for gel retardation.

These templates contain both T7 promoter sequence and polyA sequence.

Total RNA was reverse-transcribed using superscript II reverse transcriptase (Invitrogen, Carlsbad, CA), and 2 uL of the product were used as templates for multiplex PCR containing both target vimentin and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) primers for normalization.

Expression from both AUG codons was reduced, indicating that both translation products were derived from mRNA templates that contain both AUG codons.

We and others initially demonstrated that double-strand breaks generated by Cas9/sgRNA ribonucleoprotein complexes can be repaired from a plasmid harboring a DNA template containing homology on both sides flanking the lesion (Chen et al. 2013; Dickinson et al. 2013; Tzur et al. 2013; Kim et al. 2014).

For example template d which contains both Gln and Glu codons often gives significant full length peptide when Glu is withheld; we expect that this results from contaminating Glu in the Gln stock.

Using template e which contains both initiator and elongator methionine codons, we found several methionine analogs that caused defects in initiation as evidenced by the production of peptides that lacked the N-terminal Met analog altogether, and appeared to either incorporate histidine in place of methionine, or to initiate at internal sites.

The libraries were generated using a single human framework with a template containing human consensus complementarity-determining regions (CDRs) [ 62] and the antibodies that cross-react with both human and murine NRP were generated.