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For inter-subject registration, these affine-aligned GM and white matter images were warped to a study-specific tissue template constructed from all study participants using a diffeomorphic non-linear image registration algorithm [26].
Accordingly, the AluScanCNV method developed by us to call CNVs from AluScan and other types of sequence data, based on Geary-Hinkley transformation of read-depth ratios between either paired test-control samples or between test samples and a reference template constructed from reference samples [ 15], was employed in the present study for the identification of CN-gains and CN-losses.
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The filling behavior can be monitored dynamically by tracing the current transients, and the optimum conditions for filling the interstitial spaces of templates constructed from colloidal particles with 180 nm and 300 nm diameter can be obtained by applying a voltage of −5 V and −15 V, respectively.
Small volume correction was performed with templates constructed from the aal (automated anatomical labeling) toolbox [24], except for the PAG, for which the seed voxel from [9] was taken.
The DAG used as alignment template is constructed from these sequences only.
The infant template was constructed from brain scans of 10 3-month-old infants acquired with a 3.0T scanner (Sanchez, Richards & Almli, 2012).
Protospacer sequences were selected within the genes at the start and end of the cluster (redD and redF, respectively), and a 2 kb editing template was constructed from the 1 kb sequences immediately flanking the cluster.
Yoon et al. (2009) compared the tissue distributions obtained from normalizing an 8-year-old child's brain with an age-specific template (i.e., a template constructed based on 8-year-old children) and with an adult template.
Response templates were constructed from the mean local waveforms of 50 controls used in previous mfERG research [ 17].
Since the template image was constructed from adults, we checked to see if there was a potential confound of aligning adolescent brain images to that template.
The control probe template was similarly constructed from a 300 bp fragment starting 100bp upstream of the transcription start site of the pEGFP-C1 vector.
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