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This template is coregistered to a specially designed tracer-specific 18F-[FDG] template constructed by scanning 17 normal subjects in resting condition during tracer uptake in a dark room.
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We demonstrate that the system can be primed directly with SITS containing templates constructed by overlap extension PCR.
The CCHMC2 template was constructed by normalizing the brains of 200 children aged 5 to 18 years old to the MNI template.
Secondary structure modeling was performed from a template model constructed by putative conserved domains associated with the primary sequence of the novel peptide.
For each PTM analysis, gene expression profile templates were constructed by designating relative gene expression ratios for each exposure condition.
A non-HSV gene template (pSV110) was constructed by taking the plasmid pSVβgal (Promega) and inserting the HSV-1 origin of replication (oriS) SmaI fragment [ 5].
A second generation of template plasmids was constructed by adding sequences encoding one or two artificial transcription terminators upstream of the I-SceI site.
NAD-/FAD-binding residue templates were then constructed by identifying binding residues through the ligand-binding database BioLiP.
All nine SAGE libraries were constructed by template switching [ 28] using oligo-d(T) priming and each library was sequenced to a depth of greater than 20,000 tags.
To amplify array elements from genes containing only small exons (<250 bp), we used pools of cDNA libraries as a template ([ 37, 38]; pREP3X: constructed by B. Edgar and C. Norbury; Clontech).
The 26-mer G[8,5-Me]T template, 5′-GTGCG^TGTTTGTATCGCTTGCAGGGG-3′, was constructed by ligating a 14-mer to the G[8,5-Me]T cross-linked 12-mer, followed by purification.
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