Sentence examples for template confirmed that from inspiring English sources

PCR amplification with these primers using different DNAs as template confirmed that each primer pair amplified only the isoform for which it was designed, without any cross-amplification, using as template either total cDNA or specific cDNA inserted into a plasmid.

Empty vector was the single most abundant clone in the metagenomic library prior to selection, and the lack of its amplification using post-selection DNA as a template confirmed that the secretome selection step eliminated most of the "background" non-secretome-encoding recombinant phagemids, including the empty vector.

Indeed, our in vitro analyses of the binding requirements using immobilized templates confirmed that methylation of cytosine residues within the IGF2BP1 sequence does not affect CTCF binding.

Hence, the DNA phosphatase treatment restored the signal of DNA depurination which was nearly absent from previous tSMS analyses of ancient DNA templates, confirming that modification of 3'-ends, in particular phosphorylation, precluded efficient poly-A tailing of ancient DNA templates with terminal transferase.

Controls for both genomic DNA contamination of RNA (cDNA controls lacking reverse transcriptase) and for foreign DNA contamination (no template added) failed to amplify, confirming that cDNA PCR products were of mRNA origin.

Nault et al. [ 64] reported such an issue when testing T. tabaci for Wolbachia; however, negative results in repeated PCR assays using the same template DNA confirmed that the infection was the result of contamination.

RT-PCR with RNA as template using GAPDH-specific primers confirmed the absence of genomic DNA within all samples and controls with no template confirmed specificity.

Indeed, controls presented here confirm that ETA templates frequently overlap known active sites.

The plot uses signals from probes corresponding to the spiked-in controls, and confirms that unprotected templates led to low signals and vice versa for protected fragments.

The forward and reverse primers were complementary to exons 3 and 6 to confirm that amplification product was synthesized from mRNA template and not from genomic DNA.

Recombination was also monitored by Southern blot hybridization (Fig 2C), thus confirming that expression of the EGFP reporter accurately reflected the extent of template recombination.