Sentence examples for template concentrations of from inspiring English sources
The predominant peaks observed for various template concentrations of Aloevera in the following ratios of 1 0, 1 0.4, 1 0.8, 1 1.2 and 1 1.6 g calcinated at 500 °C were investigated.
Transcription rates could be rapidly adjusted by varying DNA template concentrations of the repressilator plasmid.
Each plate contained duplicate standards of purified PCR products of known template concentration covering eight orders of magnitude to interpolate relative template concentrations of the samples from the standard curves of log copy number versus threshold cycle.
Each plate contained duplicate standards of purified PCR products of known template concentration covering six orders of magnitude to interpolate relative template concentrations of the samples from the standard curves of log copy number versus threshold cycle (Ct).
Each plate contained duplicate standards of purified PCR products of known template concentration covering 7 orders of magnitude to interpolate relative template concentrations of the samples from the standard curves of log copy number versus threshold cycle (Ct).
For quantification of transcript abundance, a standard curve was generated using purified rpoD PCR product over a dilution range of known concentrations and rpoD transcript quantity in cDNA samples determined by quantitative real-time PCR was used to estimate the relative amount of template concentrations of the experimental genes.
As an example, the method is applied to measure template concentration of two Barley yellow dwarf virus (BYDV; family Luteoviridae) species in doubly infected wheat plants.
Open image in new window Fig. 1 X-ray diffraction pattern of synthesized TiO2 nanoparticles calcinated at 500 °C at varying template concentration of Aloevera (0, 0.4, 0.8, 1.2, 1.6 g T).
In each reaction MgCl2 had a final concentration of 3 mM, primers (sequences can be found in Table S2) were used at 20 mM and a template concentration of 2.5 ng was used.
Annealing was performed at a final template concentration of 5 nM, with a 20-fold molar excess of sequencing primer.
In vitro strengths were determined in 5 µl TX-TL reactions at a DNA template concentration of 1 nM.
