Sentence examples for template concentrations for from inspiring English sources
As expected, the amplification results showed the exact concordance between the HPV type and the MCPC amplification profile generated from the corresponding probe set (Figure 2) despite varied Cq (quantification cycle) values among the 15 types of HPV due to differences in starting template concentrations for each type.
they achieve optimal template concentrations for each qPCR target of interest, and, 2).
Finally, the template concentrations for PPy/MIP (7 μg/ml), PTh/MIP (3.5 μg/ml), and PMeTh (0.5 μg/ml) were applied, respectively.
Initial template concentrations for both species were quantified with NanoDrop 1000 Spectrophotometer (Thermo Scientific) and the appropriate dilutions were done so equal concentrations were used in the Q-PCR reactions.
The cDNA samples were diluted 1 10 (v/v) to the final template concentration for qRT-PCR.
At 20 ng/μl there was no apparent loss of performance; to be conservative, the minimum DNA template concentration for this assay was set at 30 ng/μl.
Correlation coefficients between Ct and DNA template concentration for each TaqMan assay were calculated by regression analysis and amplification efficiency was calculated using the formula: efficiency = -1 + 10^ -1/slope).
Although dilution of template to the single molecule limit is the simplest method for populating each droplet with a single library element, one molecule in a 100 pL droplet is insufficient template concentration for generating detectable tryptic activity.
However, because of a low template concentration for STS sites and further dilution by E. coli DNA, conventional PCR-based screening may over-dilute single locus targets to such an extent that amplifications often fail.
The predominant peaks observed for various template concentrations of Aloevera in the following ratios of 1 0, 1 0.4, 1 0.8, 1 1.2 and 1 1.6 g calcinated at 500 °C were investigated.
Only a difference in the intensity of the band was observed, which may be due to the different template concentrations used for the PCR reaction.
