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DNA composition and concentration has been shown to affect TRFLP profiles [23], and because DNA template composition and concentration necessarily varies between a bulk TRFLP analysis and twenty TRFLP analyses performed on the same DNA sample following fractionation, we would expect quantitative and qualitative differences in TRF detection.
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While a thorough analysis of the influence of template composition on sequence coverage and amplification is beyond the scope of this paper, the figures illustrate that homopolymer coverage is similar for most samples across all four nucleotides and both genomes.
Template composition of test samples for each PCR group is summarized in supporting Table S4.
The segmentation framework, currently based on deformable models, uses a template solution for dynamical composition and creation of two- and three-dimensional methods.
The influence of the initial porosity of the biocarbon template on the final phase composition and morphology of the formed biomorphic MeC/C-ceramic composites is analysed.
In this study, we showed that the control of such properties can be defined from the bottom-up, using smart surface templates to modulate the structure, composition, and bio-mechanics of human transplantable tissues.
The required relative amounts of spiropyran and MAA as well as the quantity and type of cross-linker were explored by preparing polymers with different composition and testing their template binding ability in equilibrium batch-rebinding assays.
We summarize the preparation methods, the effect of mesopore templates and composition on the mesopore-structure characteristics, and different forms of these materials, including particles, fibers, spheres, scaffolds and composites.
After dissolving the polycarbonate templates in dichloromethane (CH2Cl2), the morphology, composition, and crystallinity of nanowires were investigated by means of scanning electron microscopy (SEM, JEOL JSM-6701F), transmission electron microscopy (TEM, JEOL JEM-3010), energy dispersive X-ray spectroscopy (EDS), and selected area electron diffraction (SAED).
Moreover, hybrid template characteristics could be modified by changing the CHC composition and CHCs were produced showing a mechanical behavior similar to the native ureter.
Furthermore, the success of PCR amplifications is also influenced by the nucleotide composition and secondary structure of the DNA templates.
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