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The amplified RNA shows the similar relative level of expression as unamplified RNA indicating that the PCR based cDNA amplification technique does not give rise to a misrepresentation of the original template complexity of RNA isolated from small numbers of cells.
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TTAS is independent of the content or complexity of template sequences.
With increasing complexity of the template pool, this stipulation becomes less likely.
The major handicap for the relevant OVI was the complexity of the template, which needs revision to make it user-friendly.
However, this increases the risk that certain alleles do not hybridize, which again increases with the complexity of the template (number of alleles) because of competition between alleles during the hybridisation reaction.
By pooling a genomic DNA library made from over 9,000 N-ethyl-N-nitrosourea (ENU) mutagenized F1 fish into 32 equal pools of 288 fish, each with a unique Illumina barcode, we reduce the complexity of the template to a level at which we can detect mutations that occur in a single heterozygous fish in the entire library.
In view of the complexity of multi-step template preparation and low production, it is imperative to develop simpler template-free methods for the fabrication of magnetic Co nanowires.
There are several methods which aim to reduce the complexity of the sequencing template, in particular, by reducing the representation of low-information-content repetitive sequences.
The choice of restriction enzyme depends on the complexity of the target templates [ 25].
The rational institutional logic inherent in the template does not align easily with the complexity of emergent dialogue between the clinician and patient and it seems unlikely that minor adjustments to the design of template fields would address the communication challenges that we have identified in our research.
This threshold is also well matched to ab initio calculations of template library complexity relevant to low-level mutation calling in tumor specimens.
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CEO of Professional Science Editing for Scientists @ prosciediting.com