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By offering a quantitative foundation to define pre-analytical, sample-specific molecular variables, assess template complexity, and inform input corrections that can help guard against erroneous mutation calls, QFI-PCR offers a much needed tool to help meet the challenge of accurate NGS clinical testing of FFPE tumor biopsies.
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Furthermore, the reduction in template complexity generated by the RAD approach greatly facilitates its implementation in mapping-by-sequencing approaches.
The amplified RNA shows the similar relative level of expression as unamplified RNA indicating that the PCR based cDNA amplification technique does not give rise to a misrepresentation of the original template complexity of RNA isolated from small numbers of cells.
The result indicates that the T allele cannot be amplified by the PRT4 PCR, even in the artificial condition of an extremely low complexity and high-copy template.
In addition, a refined template selection method was proposed to reduce the computation complexity and to improve the recognition ability.
Targeted detection of FFPE DNA analytes by NGS, a method that offers true digital quantification, demands a careful consideration of template library complexity to achieve reliable and accurate results.
The secondary structure of the RNA template of telomerase, TLC1, could not be experimentally determined due its complexity and large size.
Or the complexity and vulnerability".
Their linear complexity and discordant color shock.
Hitting groundwater increases complexity and cost dramatically.
Its complexity and precision are terrifying.
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