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UV/Vis/NIR titrations show that the three-component assembly of the 12-porphyrin nanoring figure-of-eight template complex displays high allosteric cooperativity and chelate cooperativity.
UV/Vis/NIR titrations show that folding of c -P12 into the figure-of-eight template complex c -P12⋅(T6)2 is a highly cooperative process.
The 10-subunit DNA polymerase III holoenzyme has been shown to bind approximately 30 nucleotides of double-stranded DNA in a primer:template complex (12).
In this case, the script checks for each protein in the group whether it, together with all its neighbors, is a superset of a template complex.
These details show that similar pattern of interactions is brought about by residues, which are distinct from those observed in the template complex.
Specifically, γ-OH-PdG was placed opposite dC at the primer template junction, and the incoming dNTP was provided to complement the template nucleotide, 5′ adjacent to the modified nucleotide (dGTP with the 5′-CXG-3′ template (complex 1) and dATP with the 5′-TXG-3′ template (complex 2)).
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As crystal structures of mismatched primer template complexes bound to DNA polymerases have not yet been described, one can only speculate about the origin of the 5mC/C discrimination.
The interface alignment accuracy is measured using the IRACC function defined in Pulim et al. (2008): (6) where iracc ij is the alignment accuracy for a pair of template complexes i, j and is defined as nalign (i, j) is the number of aligned contacts between two complexes and nmin (i, j) is the minimum number of contacts in complexes i and j.
Template (b) is mapped to query (a) by structural alignment to form query-template complex (c).
However, these fabrication methods usually require growth templates, complex instruments, or multi-step processes to obtain aligned organic micro-/nanocrystals [25, 29] and are not quite suitable for convenient, large-area commercial productions.
Analysis of the kinetics of inhibition suggests that GE inhibits RNAP at a stage subsequent to the formation of the RNAP-template complex (Sarubbi et al., 2004).
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