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This template/assign strategy is flexible, as templates can be produced from single samples to establish a master template; combinations of samples to produce a consensus template; or stochastic subsets of cells to optimize run times and the sensitivity of detection of small clusters.
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Current syntheses can be categorised as soft template, hard template and dual template (a combination of hard and soft template).
The combined activities template is a combination of the two templates described above.
Sequencing of the unamplified SL confirmed that all of the possible combinations of template random nucleotide sequences are represented.
Units of analysis were comprised of individual forms (e.g., consent form template) or a combination of forms (e.g., primary consent template with additional secondary template for genetic research), much as they would be presented to a potential research participant.
Three homology model structures for BRP44 were built using three different combinations of templates.
The emerging paradigm is that TEs may be considered as templates of pre-organized combinations of binding sequences which can then move around in the genome carrying an already prepackaged combinatorial regulatory information which can subsequently be fine tuned by local evolutionary moves (mainly point mutations) according to the specific needs of the target genes.
The libraries were screened with riboprobes synthesized from combinations of templates chosen from three Sp185/333 gene clones that included all known elements (10-010 [GEF607629element9; element pattern G2γ], 10-022 [GEF607640element0; element pattern D1α], and 2-095 [GEF607756element6; element pattern E2δ]) [ 15].
JNK1 fusion constructs, K55R substitution mutants, and V5 tag-containing mutants were created using the PrimeSTAR Mutagenesis Basal Kit (Takara Biomedical) with zebrafish JNK1 cDNA and pcDNA6/V5-His A expression vector (Invitrogen), or derivatives, as the templates and the appropriate combinations of the forward and reverse oligonucleotide primers.
The efficacy of extension from these templates was measured by determining the kinetic parameters for incorporation of the next correct nucleotide (dG) to each primer-template combination.
The presence of quantifiers accounts for a large part of this phenomenon, as it rapidly blows up the size of the actual LTL formula passed to the trace validator: 50 copies of the same template are validated, with various combinations of values for m and x.
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