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The use of engineered nucleases combined with a homologous DNA donor template can result in targeted gene correction of the sickle cell disease mutation in hematopoietic stem and progenitor cells.
In other single-marker SNP genotyping methods, where multiple primers sets designed with similar melting temperature are present in the reaction, undesirable primer-primer interactions and non-specific amplification from the genomic template can result unless careful consideration is given to primer design and assay optimisation.
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Creating a framework for course design and standardization of templates can result in online learning that is student centered while allowing the institution to scale up enrollment with quality education at the core.
However, there are also cases that strong templates hits can result in incorrect domain assignments.
Based on this data, we concluded that the presence of 25% of mutated template can lead to misinterpretation of sequencing results and may suggest that 25% of mutated template could be difficult to detect during sequencing of DNA.
Non-homologous end joining (NHEJ) and fork stalling and template switching (FoSTeS) can result in non-recurrent events.
Neither the hairpin nor the template switching mechanism can result in the 3'-end annealing to a new genomic location that is downstream.
For this polymerase, it was also shown using 2-aminopurine fluorescence that base unstacking at the primer terminus can result in template slippage that restores pairing at the primer terminus but results in a single-base deletion mutation.
However, amplification of nonspecific templates after many PCR cycles can result in additional fluorescence unrelated to any specific target.
Current workflows often require PCR amplification of target templates from mixed samples, which can result in differential amplification of sequences from some species, leading to qualitative and quantitative biases in sequence representation from target organisms in the mixture.
Errors in DNA replication due to template slippage or unequal crossing-over can result in tandem duplication (TD), producing tandem arrays (TAR) of paralog genes in close genomic proximity [ 52].
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