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Template blanks (water) and extraction controls, consisting of lysis buffer minus tissue extract, were included in every assay.
Quantitative PCR measurements were performed in triplicate; a reverse-transcription-negative blank of each sample and a no-template blank served as negative controls.
In each case, control transcriptions were run with no reverse transcriptase; no-template blanks were also included in the PCRs as negative controls.
"The landscape of Long Island seemed an open template, a blank sheet of paper," the architectural historian Alastair Gordon wrote in "Weekend Utopia: Modern Living in the Hamptons" (Princeton Architectural Press, 2001).
You will then be taken to the basic page template, a blank Facebook profile for your business.
However, it was observed that in blank no template control wells a significant CT value of 35 or above was occasionally recorded, which was eliminated in the absence of the cDNA calibration curve.
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All reactions were run in triplicate with a non-template control blank) for each primer pair to control for contamination or primer-dimers.
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EXIF blank?
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com