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This method also prevented the potential introduction of amplification-induced error and template bias associated with whole genome amplification [26].
Other alleles may have been missed due to template bias during PCR amplification [ 40] or because sample sizes were small, e.g. InvCD141 and InvCD111 in some genotypes.
Predictably, a number of fragments found in the genome map were found missing in the reference assembled genome because of template bias.
Questions of amplification-induced error and template bias generated by these methods have previously been addressed through either small scale (SNPs) or large scale (CGH array, FISH) methodologies.
By introducing the complex formation into the reaction dynamics the template bias dependence on diffusion is different qualitatively than it is experienced in the simpler model.
Because the first step of the PCR in our study is subject to dominant template bias, remnant DNA from nucleated erythrocytes may mask mammalian DNA present in mixed blood meals.
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Our sample set was made up of cDNA clones and therefore contain some 3' template biases inherent in the sequencing of cDNAs.
To implement the search for a target in visual search, the initial activation in the knowledge network is biased towards one template unit, biasing VS-SAIM's overall behaviour towards selecting the target.
The pre-processing pipeline includes: a) re-orientating images to the standard (MNI) template, b) bias field correction, c) registration to the MNI template using both linear (FLIRT) and non-linear (FNIRT) registration tools and d) brain extraction.
Second, we profiled two intergenic negative control regions which showed negligible amplification in both H3K4me3 and mock ChIP samples, showing that there was no template loading bias in the H3K4me3 samples.
The MR images were co-registered to a standard T1-template image, bias corrected, segmented using a T1-weighted MRI template from SPM5, and spatially normalized to Montreal Neurological Institute (MNI) space (Risacher et al., 2009).
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