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De novo RdRp activity of purified NS5BΔ21s was examined by incorporation of radiolabeled GMP on homopolymeric (polyC) template (average length of 300 residues).
The homopolymeric (polyC) template (average length of 300 residues) was used to analyze the kinetic constants Vmax and Km for GTP.
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Reactions were performed in triplicate for each template, averaged, and normalized to expression of the Gapdh housekeeping gene (Mm03302249_g1).
where T m (k), similarity data; (X( i, j )), (i,j th pixel of measurement image; (Y( i, j )), (i,j th pixel of template image;, average value of pixel of measurement image;, average value of pixel of template image.
Although hPolι produced a significant number of frameshift mutations while replicating the undamaged control template, the average hPolι base deletion rate at template positions downstream from the 8-oxodG site increased by an average of 3.0-fold (Tables S3, S8, and S9, Supporting Information).
Herein, we report the preparation of carbon nanofibers containing linear mesocage arrays via evaporation induced self-assembly method within AAO template with average channel diameter of 25 nm.
It has been found that the group using the template shown average reduction in time of about 3.39 min. This time reduction is very much of importance at the time of examination.
The resulting probabilistic maps were reported in the interval [10 100%] and superimposed on the inflated representation of a template brain (average brain).
This smoothing was done so that all subjects in this study could be displayed on a common template (an average brain as described at http://surfer.nmr.mgh.harvard.edu/) in order to perform and visualize a group analysis.
Determination of quantal analysis parameters: a template (the average response) was scaled to match the rising phase of the flash-aligned response.
The decoder first built a template of average, expected population response vectors (prv) for each frame after tone-onset, up to the frame just prior to puff onset.
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