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Q-PCR reactions were carried out in 384-well plates (Applied Biosystems) and individual reactions of 10 μl contain 5.0 μl of Fast SYBR Green Master mix (1X), 2.0 μl of each primer (0.25 μM) and 1 μl of DNA template at various concentrations.
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Using equivalent quantities of DNA extracted from MTB-infected samples as templates at various dilutions, the detection limits for LAMP and PCR were both at 10−4 dilution and 3(b)).
Figure 4 SEM images of AuNPs formed using the ZnO sacrificial template annealed at various temperatures.
XRD spectra of the (a) sputtered ZnO seed layer template annealed at various temperatures.
(b) Corresponding AuNP growth using the ZnO seed template annealed at various temperatures.
Coupling of the linear porphyrin tetramer l -P4 C8 in the presence of template T6 at various mole ratios (l -P4 C8: T6) gave mixtures of cyclic and linear oligomers, all as complexes with the T6 template.
The standard curve was generated by regression in which amplification of the template of recombinant plasmid at various concentrations showed linearity over a range of four orders of magnitude).
A precursor sol consisting of titanium isopropoxide and lithium acetate was impregnated into the void space of template, and it was calcined at various temperatures.
We designed a series of donor and acceptor template pairs with either minimal pause sites or with pause sites at various locations along the template.
You can look at various websites for a template.
Total silkworm RNA harvested from silkworms at various stages of development was used as a template for RT-PCR with primers surrounding the exon 3.
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