Sentence examples for template assay from inspiring English sources
Thus, this immobilized template assay has the potential to study activating versus repressing activities concomitantly.
Using the immobilized template assay, we find that endogenous and exogenous H1 bind to the chromatin-assembled model promoter template, and that exogenous H1 potently represses transcription.
In addition, using Drosophila embryo nuclear extracts and a dual template assay system, Kadonaga has demonstrated that each round of transcription requires complete assembly and disassembly of the PIC.
Using the immobilized template assay, and binding conditions designed to precisely recapitulate the transcription reactions shown in Figs. 2a and 3e, we found that H1 supplied by the nuclear extract, or rH1 added exogenously to the reaction, directly bound the chromatin-assembled promoter template (Fig. 4a, b, lane 1).
To further define the contribution of motifs Y and Z to CTCF binding, we measured their ability to recruit CTCF in vitro using immobilized template assays.
These immobilized template assays also allowed us to determine whether those basal transcription factors travelling with RNApII in Ctk1-deficient cells remain stably associated with polymerase after it runs off the template.
(A ) Two-template assay to test the template commitment of GTFs.
In brief, oligo dT ·poly rA) primer-template assays were performed in the presence of radiolabeled [α-32P]dTTP and Mn2+.
In this regard, the important role of CBP in CREB-dependent gene expression has been demonstrated using a novel in vitro chromatinized template transcription assay.
LAMP oligonucleotides FIP and BIP (40 pmol), LB and LF (20 pmol) and F3 and B3 (5 pmol), were added together with 10 ng or 2 μl template DNA (assay screening or sensitivity/field sample testing respectively).
Taken as a whole, using the manufactured molecule template, the assay had a final sensitivity of approximately 36 synthetic genome copies per reaction, which equates to 144 genome copies per mg of tissue.
