Sentence examples for template as previously from inspiring English sources

Genomic DNA was extracted from Mtb H37Rv in the logarithmic phase and used as PCR template, as previously described (van Helden et al. 2001).

These analyses revealed that influenza transcriptase is able to use leaders from an exogenous mRNA source with a preference for leaders harboring base complementarity to the 3′-ultimate residues of the viral template, as previously observed during in vitro studies.

Polymerase activity of NS5BΔ21-fluorescence proteins was determined using LE19 RNA as template, as previously described [14].

TSHR mutants were constructed by PCR mutagenesis using the human TSHR-pcDNA3.1/hygro as a template as previously described [72].

ptch2 (formerly ptc1) RNA probe was prepared from template as previously described (Concordet et al., 1996).

An antisense mRNA probe against Pdyn was made from a cDNA template as previously described (Krawchuk and Kania, 2008).

DLS and zeta potential analysis were performed on Nano-Zetasizer (Malvern Instruments Ltd .. MSNs with a uniform diameter of ∼80 nm were synthesized using the soft template method as previously described in the literature.

ROIs were placed on the anterior cingulate cortex, striatum, thalamus, hippocampus, cerebellum, frontal cortex, temporal cortex, occipital cortex, and pons-medulla using PMOD software, with reference to an MRI template as described previously [24].

The sensitivity of each RT-PCR primer pair was determined using known quantities of a synthetic calibrant RNA template as described previously [42].

After optimizing primers efficiencies, residual primer bias was corrected by calculating the average difference in CT values between each optimized IT4 var primer pair and adenylosuccinate lyase using genomic DNA as template, as described previously [46].

Polymerase chain reaction amplification was performed with sodium bisulphate-treated DNA as template as described previously (Herman et al, 1996).