Sentence examples for template as negative from inspiring English sources

In each experiment, positive control was carried out as the standard genomic DNA along with sample run without DNA template as negative control element.

To detect genomic DNA contamination, all analyses were also carried out without reverse transcriptase and without template as negative controls.

Each PCR run also included 3 samples without RNA template as negative controls as well as a consistent triplicate calibrator comprising a mixture of multiple mouse cerebellum RNA as template.

Ultra-pure water was used replacing the DNA template as negative control.

All reactions were carried out with no template as negative controls.

The protocol for the PCR condition was: 94°C for 45 s, 53°C for 45 s, and 72°C for 60 s, with a cycle number of 32, and without DNA template as negative controls in each run.

Positive (fat body and hemocytes as template) as well as negative controls (no cDNA-synthesis) were always included.

Samples without template served as negative controls.

The reaction mix without template served as negative control and beta actin served as positive control.

The authors used total genomic DNA isolated from zebrafish cells as a positive control, and no RNA or DNA template as a negative control.

We also performed PCR using the total RNAs as template as a negative control of PCR to exclude the plasmid contamination during the RNA extraction from tumor tissues.