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The wip38,39 locus was amplified from a Wip4 template as either a 1287-bp fragmenthatat includes 258-bp of promoter sequence or a 965-bp fragment that encodes no promoter.
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Therefore, in our mapping protocol, we score the specific templates as either present (amplifiable) or absent (non-amplifiable) as a function of endonuclease-digestion time (Fig. 5 and Supplementary Fig. S1).
This finding questions the interpretation of dilution series in which template as well as non-template concentrations are simultaneously decreasing.
I think that Space Beaver comic has served as a template as far as the kind of comics I'd like to create.
The topology confirms the selected template as well as predicted 3D structure.
An identical procedure was performed to create a noise (N) template as well.
Positive (fat body and hemocytes as template) as well as negative controls (no cDNA-synthesis) were always included.
This can be found on the template as well.
Adjust the template as needed as you apply it to your flash or light source.
Make as many more using the card template as are needed.
Two microlitres of each RT product (1 : 10 000 diluted) was also used as template in either single plex or multiplexed pre-amplification reactions.
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CEO of Professional Science Editing for Scientists @ prosciediting.com