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Make as many more using the card template as are needed.
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To test whether short homology arms can support the integration of larger edits, we used PCR fragments as repair templates, as is standard in yeast (Horecka and Davis 2014), except that we did not use a selection marker.
The second, Sensitive Melting Analysis after Real Time - Methylation Specific PCR (SMART-MSP) [ 7], utilizes primers designed to amplify only methylated templates as is the case for MSP and MethyLight.
The screenplay, courtesy of Maria Melnik and Bragi F. Schut, doesn't quite operate on a "And Then There Were None" template, as it's never certain whether each encounter will result in victory, injury or death.
It has also been suggested that G-quadruplexes forming on the coding or non-template strand, as is the case with the C9orf72 repeats, could enhance transcription by keeping the template strand single stranded26.
Most news websites have a rigid layout, with stories poured into an inflexible template, as they were in the newspapers of 100 years ago.
There is no template, as he says, to being English or African: you make it up as you go along.
Fourteen questions for "nuclear energy" by using question templates, as there was only one hyponym.
Uninfected McCoy cell cDNA and water as template were used as negative controls in each assay.
Controls consisting of genomic DNA as template and no nucleic acid (water as template) were included for each primer pair.
For template-guided assembly, existing surface atomic pattern or nano/micro features as templates are made use of.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com