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It is also possible that these pauses on WT template are caused by yet unknown interaction of RNAP with nucleic acids scaffold (such as interactions of RNA exit channel with the transcript), which is altered by introduction of the ST sequence.
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Chimaeric sequences are caused by DNA template switching between PCR cycles.
This drop in the template melting temperature is caused by the excessive presence of T and A bases in the RRBS libraries.
In individual cases, the similarity to the given template can also be caused by experimental inaccuracies or noise which are both known to be rather high in microarray experiments.
This improvement in the light output power of the LED with the PSS-ANP template that had been etched for 20 min is caused by the thermal conductive effect of the void in the template structure.
Moreover; the amorphous background in the pattern is caused by the AAO template.
A further limitation is caused by the use of the standard SPM template for adults for spatial normalization in children.
PCR primer bias is thought to be caused by inhibition of amplification by self-annealing of the most abundant templates in the late stages of amplification [ 50] or as a result of differences in the amplification efficiency of templates [ 51].
However, in any PCR-based typing scheme, typing failure may be caused by primer template mismatch, which could bias results, especially with novel strains (16, 38 ).
Mismatches of primers with the targeted region of the DNA templates are thought to be the cause of differences in the estimation of the relative abundance of bacterial species in environmental samples.
The template is there.
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