Exact(2)
PCR amplification was performed in 50 μL reaction containing 1X buffer, 2 mM MgCl2, 0.12 mM of dNTPs mixture, 1 μM of each primer, 5% DMSO, 1.5 U Taq polymerase, and 10 ng of template, applying the following thermic protocol: 94°C for 4′, followed by 30 cycles of 94°C for 45′′, 55°C for 1′, and 72°C for 1′, and a final extension at 72°C for 10 min.
PCR amplification was performed in 25 μL reaction containing 1X buffer, 1.5 mM MgCl2, 0.12 mM of dNTPs mixture, 0.3 μM of each primer, 1 U Taq polymerase, and 10 ng of template, applying the following thermic protocol: 94°C for 4 min, followed by 35 cycles of 94°C for 0.5 min, 55°C for 1 min, and 72°C for 2 min and a final extension at 72°C for 10 min.
Similar(57)
After these corrections, the images were spatially normalized to the standard space of the standard Montreal Neurological Institute MNII) template by applying the EPI template at a 3 × 3 × 3 mm resolution.
This can be designed by various approaches, e.g.: (i) using a natural ligand-protein complex as a template; (ii) applying the principle of complementarity to exposed residues of the protein structure; and (iii) mimicking directly a natural biological recognition interaction.
Then, these models with the crystallographic structures suggested by BLAST were used as template for applying the comparative modelling strategy in order to obtain the complete models for both regions.
Instead of using the template structure of the model for the rescoring we take all structures in the fold of the template, apply VORPSI, and take the best score among these templates.
The Keypress template applies specific CSS style to emulate the look of a keyboard key.
The pseudo CT is generated by non-rigidly registering the segmented patient's T1 image to a segmented T1 template and by applying the inverse transformation to the associated CT template.
The article will automatically have the appropriate NFD template applied to it, and will be demoted (removed from the NAB queue).
Individual PET images were spatially normalized into the space of a predefined mouse brain template [ 43] by matching the individual animal specific CT to the template CT and applying the same transform to the PET which is by default matched to its CT via the PET/CT scanner hardware.
To test for regional hypoperfusion, the PWI images were normalized to the T1-weighted template space by applying the same transformation parameters than for the T1-weighted images.
Write better and faster with AI suggestions while staying true to your unique style.
Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com