Sentence examples for template and with from inspiring English sources

Control reactions were conducted with no cDNA template and with RNA to determine the level of background or genomic contamination.

The individual fluorescent proteins were amplified by PCR with pmTFP1 or mVenus as a template and with the following two primers 5′-GATCAGTACTAATGGTGAGCAAGGGCGAG and 5′-TGATCGCGGCCGCTCTTGTACAGCTCGTCCATGC.

The mTFP1-5aa-Venus gene was amplified by PCR with pmTFP1-5aa-Venus as a template and with the following primers: 5′-CGCTACCGGTCGCCACCA and 5′-GATCGCGGCCGCTTAGTACCGTCGACTGCAGAATTC.

Each model was aligned both with its respective template and with a fourth template that also fits the LeuT/vSGLT/Mhp1 fold (BetP).

First, the promoter (P) and terminator (T) regions were amplified from Y. lipolytica W29 genomic DNA as the template and with the gene-specific P1/P2 and T1/T2 oligonucleotides as primer pairs (Additional file 6).

The real-time PCR reaction was performed in a total volume of 25  μl (22  μl Master Mix and 3  μl DNA template), and with the use of optical 96-well micro-plates were used.

The revocability is provided to the cryptographic key with cancelable template and or with updated shuffle key.

In each qPCR reaction two negative controls were included, one with no cDNA template and one with no reverse transcription treatment.

Primers were first tested with genomic DNA as a template, and then with whole ovary cDNA as a template (primers and conditions available upon request).

An oligonucleotide with sequence GGCCCGGG was annealed to tagrep template and extended with DNA polymerase Klenov fragment.

Second, for each gene PCR amplification was performed with equal amounts of purified fragments #1 and #2 as templates and with no additional primers, yielding the fusion genes mamK-venus and mamK-like-venus.