Sentence examples for template and vector from inspiring English sources

Properties of Read.pm include e.g. the DNA sequence, quality scores, template and vector names and read direction.

Putative transformants were screened by PCR analysis using tobacco genomic DNA (from WT and various transgenic lines) as template and vector specific primers (forward primer 5'-CAAGACCCTTCCTCTATATAAG-3' and reverse primer 5'-CAAGAATTGGGACAACTCCAG-3').

The resulting pressure template and latency vector are compared with those modeled in the database using the AR and latency classifiers.

In Sections 4 and 5 we define two additional problem templates: the Outside Vector Multiplication Template, and the Multiple String Vector Multiplication Template, and describe modifications to the algorithm of Valiant which allow to solve problems that sustain these templates.

Each PCR primer consisted of an ∼20-nucleotide (nt) sequence that annealed to the vector template and an ∼30-nt overhang that overlapped with one end of the target DNA.

After PCR, these products were pooled, digested with Dpn1 for 4 hr at 37°C to remove vector template, and concentrated by isopropanol precipitation.

Use 2μl of pSilencer with the siRNA template and a pSilencer control vector with no insert to transform DHα5 E. coli (Bio S&T, Montreal, QC) according to manufacturer's protocol onto LB plates containing 50μg/ml of ampicillin.

The LBD swap constructs were created by amplifying the corresponding LBD coding regions from C. briggsae fax-1, nhr-67, and nhr-111, using corresponding cDNA clones as template, and ligating each to vector pGEM-T Easy using oligonucleotides tailed with BclI, BamHI and/or EcoRI recognition sites (Additional file 3: Table S1).

The vector amplification reaction mixture contained 5 μL of 10× KOD buffer, 0.2 mM dNTPs, 2 mM MgCl2, primers at 0.3 μM each (forward primer, 5′-AAC CTC TAC TTC CAA TCG CAC CAT CAT CAC CAC CAT TG-3′; reverse primer, 5′-TAT ATC TCC TTC TTA AGG TTA AAC AAA ATT ATT TCT AG-3′), 10 ng of vector template, and 1 unit of KOD polymerase in a total volume of 50 μL.

To convert the BV to a gene targeting vector, a DNA donor template and ZFNs were incorporated into the vector.

In this cloning strategy, PCR amplified target gene is inserted into plasmid vector by means of a lineal recombination PCR using circular vector as template and target gene PCR products as primers, which contains two primer-introduced terminal sequences homologous to correspondent sequences in the vector.