The system is driven by the medical domain knowledge defined by clinicians in the form of templates and variable definitions stored in a common data repository.
A second important aspect of the template system is that it is a highly flexible and adaptive system since its runtime behaviour is driven by templates and variable definitions created and maintained by the end users, the medical professionals themselves.
Templates and variables can be defined during system runtime to adapt the system to meet new requirements on data recording.
Requirements unforeseen by the software developers can be easily accomplished by creating new or updating existing templates and variables.
The TaqMan PCR reaction mixture was assembled from a 2× ddPCR Mastermix (Bio-Rad), 20× primer, and probes (final concentrations of 900 and 250 nM, respectively) and template (variable volume) in a final volume of 20 μL.
The current Utstein Trauma Template variables and definitions could be further improved before collaborative research on the comparison of trauma care performance is initiated on a larger scale.
A propensity score for the probability of a patient to receive TH was calculated with a logistic regression model based on Utstein template variables and medical relevant interaction terms among these.
Amplification conditions for RAG1-I2 were 1X ProMega GoTaq Flexi reaction buffer, 0.2 mM dNTP's, 2 mM MgCl2, 0.4 μM forward primer, 0.4 μM reverse primer, 0.025U/μL GoTaq Flexi Taq polymerase, and 1 μL template of variable concentrations.
We chose a simple heuristic due to a lack of options, as we are not aware of any other openly available method for performing automatic pagination and template selection for variable content.
Although the quality of the DNA templates was variable and often crude, the TSP assays produced the correct genotypes from the range of DNA samples, demonstrating that DNA quality was not a limiting factor.
