Sentence examples for template and transformed from inspiring English sources

We generated a pooled library of varying length deletions within exon 2 of mrps16 by combinatorial inverse PCR and blunt ligation, using the wt mrps16 plasmid as a template, and transformed this pool of mutants into wild-type S. pombe.

As per the manufacturer's protocol, the PCR product was treated with Dpn I endonuclease to digest the methylated wild type DNA template, and transformed into Epicurian Coli XL1-Blue supercompetent cells.

The standard and parametric modeling process of LVDT is initially recorded as a script and then identifiers are added into the script to form a script template and transform Computing Aided Design CADD) parameters to CAE modeling parameters according to parameter constraints of the model.

The PCRs were treated with the restriction endonuclease DpnI to digest the template DNA, and transformed into E. coli.

Each participant's original MRI was then non-linearly registered to this tentative average template (A0) and transformed in size and orientation using the "Advanced Normalization Tools" (ANTS; Avants et al., 2008, 2011).

With the size of the transform parameter key and transformed templates being moderate, the proposed method suits resource-limited applications, e.g., smartcards, driver license and mobile phones.

All images were realigned and transformed into the standardized stereotactic reference system (EPI-template provided by the Montreal Neurological Institute).

These images were spatially normalized and transformed into a common space, as defined by the SPM Montreal Neurological Institute (MNI) T1 template.

The PDS utilizes the template definitions published by TDS and transform the template into web forms ready for data entry.

A study-specific mean image template was reiteratively created, and the final template was affine transformed to the template defined by the Montreal Neurological Institute (MNI).

The PCR product, thus obtained was purified, digested with DpnI restriction enzyme to cleave the methylated template DNA, transformed into E. coli DH5α strain and plated on LB agar media containing ampicillin (50 µg/ml).