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Pathologic sampling was performed using the grid as a template, and tissue blocks containing DCIS were reported on the grid.
An integrated approach (unified segmentation, Ashburner and Friston, 2005) was used for bias correction, image registration to the Montreal Neurological Institute MNII) template and tissue classification into grey matter, white matter and cerebrospinal fluid.
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To explore its potentials in nanolattice templating and tissue scaffolding, this work focused the viscoelasticity of the superlattice with a novel transient method via atomic force microscopy (AFM).
To account for potential differences between our local sample and the templates provided in SPM2, an "optimized VBM" protocol was used, as described in Good et al. [ 67], which results in the use of a study-specific anatomical template for spatial normalization and tissue segmentation, as described below.
Another important requirement of tissue engineered systems is the scaffold, which has to be able to sustain and promote the growth of the relevant cells and provide a temporary template for tissue growth and incorporation with the host tissue.
It is widely accepted that three-dimensional porous constructs are needed to lodge bone cells and to provide the template for tissue formation and development, including angiogenesis.
Regardless of the specific tissue engineering strategy, the presence of a scaffold is essential to provide a three-dimensional template for tissue growth and cell adhesion and to maintain the mechanical stability of the tissue defect.
A full-thickness circular wound measuring 15 mm in diameter was marked to the right of midline using a standard template, and the skin, subcutaneous tissue and fascia were excised.
Here, the scaffold serves as a physical support structure for cell growth and a template for tissue regeneration.
Scaffold is crucial in tissue engineering as a delivery vehicle for cell transplantation, a three-dimensional template for tissue regeneration, and as a matrix for signal transduction to regulate bone formation (Harris et al.,1998).
The whole preprocessing routine included skull stripping, custom template creation, tissue classification and normalization.
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