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To detect the level of genomic DNA still present in the 24 RNA samples after the DNase I treatment, qRT-PCR was performed using 7.5 ng of isolated RNA as template, and three different primer pairs listed in Supplementary Table S2.
The WT template and three clones (D112G-1, D112G-3 and D112G-5) lost detectable products at 68°C, whereas the other two (D112G-2 and 4) maintained detectable over 68°C; this annealing temperature was therefore identified as the Tc of D112G.
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The probabilities of forming non-covalently bonded complexation between the template and five commonly used monomers were evaluated by electronic interaction energy.
Using the 5′-RACE-Ready cDNA of lemon as a template and two degenerate primers (5′ AGY CAA GGT GCH TTC CAG 3′ and ACY TTM ACW GGC TCR TTG TT), a 0.2 kb fragment was amplified by PCR.
Nitrogen-doped ordered mesoporous carbons (N-OMCs) were synthesized by nanocasting using SBA-15 silica as template and two polyaramide precursors: m-aminobenzoic acid (MABA) and p-aminobenzoic acid (PABA).
Employing netropsin (NT) as a template and nine different 2-aminothiazole analogues have been examined for their capacity to interact with the DNA structure based on the number and pattern of H-bonding, energy of binding, conformational changes of compounds due to docking, and the width change of DNA minor groove.
(encoded by p369-c1, GenBaccessionsionumberer GU994007) was mutagenized by "divergent PCR" using p369-c1 (MethodS1S1) as a template and one of two forward primers containing 5'-NBR or 5'-NVN extensions and a juxtaposed reverse primer (Table S2).
A gray cue template and two Gabor patches colored low-contrast green and red were projected onto a screen, which the participants viewed via a mirror mounted on the head coil of the MRI scanner.
The DNA sequence of each transposon insertion site was determined using the mutant chromosomal DNA as the template and two different sequencing primers that bind within the transposon sequence (primers are listed in Table 2).
14028S (gi: 267990107) as a template and two primers: forward TraJA and backward TraJB.
Three structures were generated, one using [PDB 1CEN] as template and two using the different chains in [PDB 3AMG] as templates.
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