Sentence examples for template and target from inspiring English sources
On the basis of major mutation in VP28 isolated from Bangladesh, the protein modeling was performed taken PDB id 2ED6 (Tang et al. 2007) and BAN_SH_BG-1C_2014 as template and target, respectively.
In this cloning strategy, PCR amplified target gene is inserted into plasmid vector by means of a lineal recombination PCR using circular vector as template and target gene PCR products as primers, which contains two primer-introduced terminal sequences homologous to correspondent sequences in the vector.
Firstly, ClustalW was used to create an automatic alignment of all of the template and target GPCR sequences [67].
Comparative homology modelling is, however, challenging when sequence identity of the template and target is <30% [21], [22].
The multiple sequence alignment (MSA) of the template and target GPCR sequences was produced using a two tier approach.
In general, the average homology model accuracy is a function of the template-target sequence similarity: The dispersion of the model-target structural overlap increases with the decrease in sequence identity between template and target.
Templates and target sequences were aligned using Promals3D [ 42] and manually optimized with the support of the DNATagger [ 43] by monitoring the alignment of cysteine residues and conservation of physicochemical properties of aligned residues.
To ensure reproducible and robust aCGH assay quality, we developed methods and protocols using the Agilent BioAnalyzer (Agilent Technologies, Palo Alto, CA, USA) to enable accurate quality control for key prehybridization steps, including: phi29 amplification of genomic samples, restriction digestion of templates and target labeling.
For the fabrication of closed circular DNA, the template DNA and target DNA were mixed at the final concentration of 2.5 μM.
Unique identifiers between template sequence and target sequence were compared to determine homology within the sequence by using multiple sequence alignment available in Command Line Calculator (CLC) drug discovery workbench.
The method uses an effective consensus strategy by combining PSI-BLAST, IMPALA, and T-Coffee in both template selection and target-template alignment.
