The pool screening was done in a two-round PCR procedure by first using super pools (12 plates) as templates and second using matrix pools as template following the User's Manual version 3.2 (Amplicon Express, Pullman, WA 99163 USA).
Then, the normalized cross correlation between the template image and second image is calculated while the template image is shifted all along the search window.
CFTR constructs were screened for reactivity toward both channel-permeant and channel-impermeant thiol-directed reagents, and patterns of reactivity in TM6 were mapped onto two new, molecular models of the CFTR pore: one based on homology modeling using Sav1866 as the template and a second derived from the first by molecular dynamics simulation.
Unfortunately for the small players in this space, templates and third-party services are often how they enter the App Store.
In the present study, we compared two methods for AV-45 cortical quantification, the first one quantifying regional mean SUVr measured using an AV-45 template, and the second an optimized method based on cortical signal extraction of SUVr using individually segmented T1 MRI sequences.
The first stage utilized a standard 12 degrees of freedom registration to the template and the second stage applied 12 degrees of freedom registration using an MNI152 subcortical mask to exclude voxels outside the subcortical regions.
In a variation of this method, one long oligo is used as the template and a second short oligo (generic) is used as the primer for extension (Fig. 1d and Additional file 2).
The first entry clone also harboured the NLS from the SV40 large T antigen and was made by two rounds of PCR, the first using a sense primer incorporating the NLS and wild-type or mutant pcDNA5/FRT/TO-eGFP-DEST/CREBBP described above as the template and the second to add the appropriate attB sites to the PCR product, followed by BP cloning into the pDONR221 P1-P4 vector.
Red curve with stars: correlation values between the template and the first derivation curve in function of the position of the template.
The invading ssDNA 3′-ends undergo DNA synthesis using the homologous duplex as a template and after second-end capture forms double Holliday junctions (dHJs) (Szostak et al., 1983; Schwacha and Kleckner, 1995).
