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After assigning cells from different samples to the cluster template, the medians of the resulting clusters were recalculated and compared with the medians of the original template, i.e., testing the "goodness of fit" between template and sample.
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qPCR was carried out as described above. 1 μL of the 20 μL cDNA reaction was used as template and samples were run as quadruplicates.
For example, the Lasallian Education section has the Lasallian Guiding Principles of Education, while the section on Curriculum has templates and sample syllabi as well as other references for writing the learning outcomes.
For example, the Web site of the American College of Radiology Imaging Network provides access to standard enrollment form templates and sample protocols [ 33].
JNR and DRA provided project and diagnostic design/development and performed diagnostics, JDW conceived the project and provided project design and management, MSS performed template preparation and sample management, RD and SR collected blood and information on the animals.
The Society of Human Resource Management has numerous templates and samples, and some data on startup policies is covered here.
Then it updates haplotype configurations by using the current set of haplotype estimates for all individuals as templates, and sampling S using the Markov Chain.
SL sample (S1) was grown on a GaN 5 μm -on-sapphire template, and SL sample (S2) was grown on an AlN(340 nm)-on-sapphire template.
Negative controls included a reagent control (sterile water served as PCR template) and a sample preparation control (sterile water used in place of the original sample and exposed to the entire extraction protocol).
Mycobacterial DNA, and positive PCR product were included as positive controls and an extraction control (with all the steps but without any tissue), a reaction tube with substitution of distilled water for the test template and a sample which previously yielded negative result on PCR were included as negative control in each PCR run.
Each run consisted of six external standards, a negative control without a template and patient samples with unknown mRNA concentrations.
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